使用aimhii跑外源插入时报以下错误：
#python aimhii -r bwa.fa --maxgap 5000 --outfile results.csv --fqtype illumina --threads 10 -t aimhii_output --plot readplot Zea_mays.dna.toplevel.fa adapter.fa 506DNA_1.clean.fq.gz 506DNA_2.clean.fq.gz
/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/site-packages/Bio/__init__.py:128: BiopythonWarning: You may be importing Biopython from inside the source tree. This is bad practice and might lead to downstream issues. In particular, you might encounter ImportErrors due to missing compiled C extensions. We recommend that you try running your code from outside the source tree. If you are outside the source tree then you have a setup.py file in an unexpected directory: /share/work/biosoft/python/Python-v2.7.11/lib/python2.7/site-packages.
format(_parent_dir), BiopythonWarning)
--------------------TRIMMING READS--------------------
--------------------JOINING READS--------------------
Traceback (most recent call last):
File "/share/work/biosoft/python/Python-v2.7.11/bin/aimhii", line 11, in <module>
sys.exit(main())
File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/site-packages/aimhii/__init__.py", line 77, in main
joined_filenames = run_fastqjoin(trimmed_fastqs,tmpdir)
File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/site-packages/aimhii/__init__.py", line 153, in run_fastqjoin
subprocess.check_output(cmd, preexec_fn=lambda: signal.signal(signal.SIGPIPE, signal.SIG_DFL))
File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/subprocess.py", line 566, in check_output
process = Popen(stdout=PIPE, *popenargs, **kwargs)
File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/subprocess.py", line 710, in __init__
errread, errwrite)
File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/subprocess.py", line 1335, in _execute_child
raise child_exception
OSError: [Errno 2] No such file or directory
原因是 PATH环境变量缺少 ea-utils 软件的路径：/share/work/biosoft/ea-utils/bin，将其加到环境变量即可。
EA-utils是一个用于Illumina测序数据分析的开源软件包。它包含了一系列命令行工具，可以用于质量控制、去除低质量序列、去除接头、序列比 对等一系列数据分析任务。
