问题：
在用BWA进行序列比对时出现：[mem_sam_pe] paired reads have different names: "A00920:973:H5GWJDSX3:2:1103:2582:12633:UMI_AAT_GTA", "A00920:973:H5GWJDSX3:2:1103:1624:12633:UMI...
问题：
在用BWA进行序列比对时出现：[mem_sam_pe] paired reads have different names: "A00920:973:H5GWJDSX3:2:1103:2582:12633:UMI_AAT_GTA", "A00920:973:H5GWJDSX3:2:1103:1624:12633:UMI_CGG_GTA"
相同行出现非paired reads情况，导致比对出错
问题解决：采用bbmap进行fastq文件的修复
1.软件安装
conda安装
conda install -c bioconda bbmap
或直接安装
wget https://nchc.dl.sourceforge.net/project/bbmap/BBMap_39.01.tar.gz
2.fq文件修复
sh ./repair.sh in=r1.fq in2=r2.fq out=r1.repair.fq out2=r2.repair.fq
参考：https://blog.csdn.net/weixin_47621318/article/details/127238717
