#去掉序列ID中注释信息,避免错误:no ASV ids overlap between input FASTA and sequence abundance table
get_fa_by_id.pl $workdir/5.pick_otu_qiime/pick_de_novo_otus/otu_table_clean.txt $workdir/5.pick_otu_qiime/pick_de_novo_otus/rep_set/qiime_rep_set.fasta rep_set.fa
rm -rf picrust2_out
picrust2_pipeline.py -s rep_set.fa -i $workdir/5.pick_otu_qiime/pick_de_novo_otus/otu_table.biom -o picrust2_out --processes 1 --in_traits COG,EC,KO,PFAM,TIGRFAM
add_descriptions.py -i picrust2_out/EC_metagenome_out/pred_metagenome_unstrat.tsv.gz -m EC \
-o picrust2_out/EC_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i picrust2_out/KO_metagenome_out/pred_metagenome_unstrat.tsv.gz -m KO \
-o picrust2_out/KO_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i picrust2_out/COG_metagenome_out/pred_metagenome_unstrat.tsv.gz -m COG \
-o picrust2_out/COG_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i picrust2_out/PFAM_metagenome_out/pred_metagenome_unstrat.tsv.gz -m PFAM \
-o picrust2_out/PFAM_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i picrust2_out/TIGRFAM_metagenome_out/pred_metagenome_unstrat.tsv.gz -m TIGRFAM \
-o picrust2_out/TIGRFAM_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i picrust2_out/pathways_out/path_abun_unstrat.tsv.gz -m METACYC \
-o picrust2_out/pathways_out/path_abun_unstrat_descrip.tsv.gz
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