安装方法：

All installation instructions are in more detail here.

First, clone the repository and enter the directory:

git clone https://github.com/picrust/picrust2.git
cd

Next, install the required dependencies listed here.

Install PICRUSt2 into it's own environment using conda:

conda env create -f picrust2-env.yaml
source activate picrust2
pip install --no-deps --editable .

Finally, run the tests to verify the install was successful:

pytest

测试报错：

Standard error of the above failed command:

Traceback (most recent call last):

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/bin/picrust2_pipeline.py", line 4, in <module>

    __import__('pkg_resources').require('PICRUSt2==2.3.0b0')

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", line 3267, in <module>

    @_call_aside

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", line 3251, in _call_aside

    f(*args, **kwargs)

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", line 3280, in _initialize_master_working_set

    working_set = WorkingSet._build_master()

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", line 582, in _build_master

    ws.require(__requires__)

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", line 899, in require

    needed = self.resolve(parse_requirements(requirements))

  File "/share/work/biosoft/conda/Miniconda3.8/envs/picrust2/lib/python3.6/site-packages/pkg_resources/__init__.py", line 785, in resolve

    raise DistributionNotFound(req, requirers)

pkg_resources.DistributionNotFound: The 'cython' distribution was not found and is required by biom-format

解决办法:

重新安装：pip install biom-format