qiime2 couldn't connect to display "localhost:12.0"

Traceback (most recent call last):
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__
    results = action(**arguments)
  File "<decorator-gen-545>", line 2, in summarize
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
    output_types, provenance)
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/action.py", line 452, in _callable_executor_
    ret_val = self._callable(output_dir=temp_dir, **view_args)
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/q2_demux/_summarize/_visualizer.py", line 177, in summarize
    ax = sns.distplot(result, kde=False, color='black')
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/seaborn/distributions.py", line 167, in distplot
    ax = plt.gca()
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/matplotlib/pyplot.py", line 879, in gca
    return gcf().gca(**kwargs)
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/matplotlib/pyplot.py", line 611, in gcf
    return figure()
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/matplotlib/pyplot.py", line 546, in figure
    **kwargs)
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/matplotlib/backend_bases.py", line 3358, in new_figure_manager
    return cls.new_figure_manager_given_figure(num, fig)
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/matplotlib/backends/_backend_tk.py", line 888, in new_figure_manager_given_figure
    window = tk.Tk(className="matplotlib")
  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/tkinter/__init__.py", line 2023, in __init__
    self.tk = _tkinter.create(screenName, baseName, className, interactive, wantobjects, useTk, sync, use)
_tkinter.TclError: couldn't connect to display "localhost:12.0"
Plugin error from demux:
  couldn't connect to display "localhost:10.0"
Debug info has been saved to /tmp/qiime2-q2cli-err-cpq2ii3g.log

【出现问题的原因】问题在于，您使用的是一个交互式后端，它试图为您创建图形窗口，但由于您断开了启动模拟时可用的x服务器，所以失败了。

【解决方法】

使用非交互式后端(请参见后端？)比如：Agg(用于Png格式，PDF, SVG或PS。在生成图形的脚本中，只需在import matplotlib.pyplot as plt之前调用matplotlib.use(）即可，具体如下：

将导入更改为

import matplotlib
matplotlib.use('AGG')#或者PDF, SVG或PS
import matplotlib.pyplot as plt

永久解决办法：

mkdir -p $HOME/.config/matplotlib/ 
echo"backend: Agg" >> $HOME/.config/matplotlib/matplotlibrc

发表于 2021-01-04 15:29
阅读 ( 4312 )
分类：宏基因组

qiime2 couldn't connect to display "localhost:12.0"

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