我们经常会看到进化树外层有圆环用以区分样品分组等,大家可能不知道怎么画,iTOL可以很好地实现这一功能。
方法很简单,首先准备好进化树。
然后准备注释文件,其格式如下:
DATASET_COLORSTRIP
#lines starting with a hash are comments and ignored during parsing
#select the separator which is used to delimit the data below (TAB,SPACE or COMMA).This separator must be used throught this file (except in the SEPARATOR line, which uses space).
#SEPARATOR TAB
SEPARATOR SPACE
#SEPARATOR COMMA
#label is used in the legend table (can be changed later)
DATASET_LABEL color_strip2
#dataset color (can be changed later)
COLOR #ff0000
#optional settings
#all other optional settings can be set or changed later in the web interface (under 'Datasets' tab)
#maximum width
STRIP_WIDTH 25
#left margin, used to increase/decrease the spacing to the next dataset. Can be negative, causing datasets to overlap.
MARGIN 0
#border width; if set above 0, a black border of specified width (in pixels) will be drawn around the color strip
BORDER_WIDTH 1
BORDER_COLOR #000
#show internal values; if set, values associated to internal nodes will be displayed even if these nodes are not collapsed. It could cause overlapping in the dataset display.
SHOW_INTERNAL 0
#In colored strip charts, each ID is associated to a color. Color can be specified in hexadecimal, RGB or RGBA notation
#Internal tree nodes can be specified using IDs directly, or using the 'last common ancestor' method described in iTOL help pages
#Actual data follows after the "DATA" keyword
DATA
#ID1 value1
#ID2 value2
160232 #efff22 COL#efff22
13773 #efff22 COL#efff22
56636 #efff22 COL#efff22
111955 #efff22 COL#efff22
2287 #efff22 COL#efff22
50339 #efff22 COL#efff22
2303 #efff22 COL#efff22
2234 #efff22 COL#efff22
DATA之前的部分都是格式声明,照抄即可。之后就是添加圆环数据,数据分两列,第一列为原始标签,第二列是圆环对应颜色,之间以Ta空格分隔。准备好数据后就可以加到进化树中了。
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