5 想请教一下，如果在stringtie定量后，得到的某基因有多个转录本，那么它的定量结果，即FPKM值应该如何取舍？
FAN_iscf00000040.1 StringTie transcript 1835 3860 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; cov "13.755408"; FPKM "2.461453"; TPM "1.513562";
FAN_iscf00000040.1 StringTie exon 1835 2465 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "1"; cov "14.038827";
FAN_iscf00000040.1 StringTie exon 3559 3634 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "2"; cov "18.973684";
FAN_iscf00000040.1 StringTie exon 3736 3860 1000 - . gene_id "STRG.20"; transcript_id "STRG.20.1"; exon_number "3"; cov "9.152000";
FAN_iscf00000040.1 StringTie transcript 3634 4037 1000 + . gene_id "STRG.21"; transcript_id "STRG.21.1"; cov "3.374046"; FPKM "0.603767"; TPM "0.371260";
FAN_iscf00000040.1 StringTie exon 3634 3845 1000 + . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "1"; cov "3.287736";
FAN_iscf00000040.1 StringTie exon 3988 4037 1000 + . gene_id "STRG.21"; transcript_id "STRG.21.1"; exon_number "2"; cov "3.740000";
FAN_iscf00000040.1 StringTie transcript 3955 4286 1000 - . gene_id "STRG.22"; transcript_id "STRG.22.1"; cov "11.914286"; FPKM "2.131994"; TPM "1.310976";
FAN_iscf00000040.1 StringTie exon 3955 4075 1000 - . gene_id "STRG.22"; transcript_id "STRG.22.1"; exon_number "1"; cov "9.132232";
FAN_iscf00000040.1 StringTie exon 4163 4286 1000 - . gene_id "STRG.22"; transcript_id "STRG.22.1"; exon_number "2"; cov "14.629032";
比如上面FAN_iscf00000040.1基因有3个转录本，最后的FPKM值也有三个，像这种情况该如何取舍？谢谢！
最佳答案 2018-08-29 10:00
GFF文件格式来看，
1. FAN_iscf00000040.1 这个是染色体（参考序列），而不是基因。
2. 基因的名称应该是 “ gene_id” 后面跟着的那个属性
3. 你这个不是一个基因的三个转录本，而就是3个基因
