The predicted genes were then manually checked using NCBI and UniPort online BLASTP, with threshold of E-value < 10-25.
Identified genes were validated by aligning to a nonredundant gene dataset. Identified genes were also validated by BLAST searching against an EST database with threshold E-value <10-30, identities >90%, and match lengths >100 bp. 这个验证鉴定的基因的方法该怎么操作？ 谢谢
