请用课程指定版本的镜像:docker pull omicsclass/ampliseq-q2:v1.4
利用dada2生成特征表,输入命令(省略了部分选项):
qiime dada2 denoise-paired --i-demultiplexed-seqs $workdir/1.import_data/demux.qza --p-n-threads 2 --p-trim-left-f 29 --p-trim-left-r 18
提示: Plugin error from dada2: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more. Debug info has been saved to /tmp/qiime2-q2cli-err-u8wvbzh9.log
打开日志文件:
Error in library(BiocManager, quietly = TRUE) : there is no package called ‘BiocManager’ Calls: .First -> library Execution halted