这个具体的报错log文件贴一下:work/my_amplicon/tmp/qiime2-q2cli-err-cojm7_ll.log
可能数输入文件有问题,说序列长度不等,你看看输入文件;
根据视频课程构建16S进化树时一直提示这个
Plugin error from phylogeny:
work/my_amplicon/tmp/q2-AlignedDNAFASTAFormat-3r8bwdoj is not a(n) AlignedDNAFASTAFormat file:
The sequence starting on line 2 was length 404. All previous sequences were length 0. All sequences must be the same length for AlignedFASTAFormat.
Debug info has been saved to work/my_amplicon/tmp/qiime2-q2cli-err-cojm7_ll.log