老师你好,构建进化树16S分析一直出错

根据视频课程构建16S进化树时一直提示这个

Plugin error from phylogeny:

work/my_amplicon/tmp/q2-AlignedDNAFASTAFormat-3r8bwdoj is not a(n) AlignedDNAFASTAFormat file:


  The sequence starting on line 2 was length 404. All previous sequences were length 0. All sequences must be the same length for AlignedFASTAFormat.


Debug info has been saved to work/my_amplicon/tmp/qiime2-q2cli-err-cojm7_ll.log


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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

这个具体的报错log文件贴一下:work/my_amplicon/tmp/qiime2-q2cli-err-cojm7_ll.log

可能数输入文件有问题,说序列长度不等,你看看输入文件;

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