建议用我们的docker镜像,里面都设置好了。自己配置PASA可以参考PASA官方网站;
不同的服务器mysql安装环境不一样,我这边不好帮你查原因
运行/ldata/xiongmei/ceshi/PASApipeline.v2.4.1/Launch_PASA_pipeline.pl -c pasa.annotationCompare.Template.txt -g wjsy_rm_adj.fa -t transcripts.fa -A -L --annots evm.out.gff3,总是报错DBI connect('database=wjsy11;host=localhost','shehb',...) failed: Access denied for user 'shehb'@'localhost' (using password: YES) at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/PerlLib/DB_connect.pm line 72.
Cannot connect to wjsy11: Access denied for user 'shehb'@'localhost' (using password: YES) at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/scripts/Load_Current_Gene_Annotations.dbi line 71.
Error, cmd: /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/scripts/Load_Current_Gene_Annotations.dbi -c pasa.annotationCompare.Template.txt -g wjsy_rm_adj.fa -P evm.out.gff3 > pasa_run.log.dir/output.annot_loading.69620.out died with ret 65280 No such file or directory at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/PerlLib/Pipeliner.pm line 187.
Pipeliner::run(Pipeliner=HASH(0x1a2a6b0)) called at /ldata/xiongmei/ceshi/PASApipeline.v2.4.1/Launch_PASA_pipeline.pl line 1044