run_pipeline.pl -Xmx30G -SortGenotypeFilePlugin -inputFile $vcf \
-outputFile clean.sorted.vcf.gz -fileType VCF
run_pipeline.pl -Xmx5G -importGuess clean.sorted.vcf.gz \
-ExportPlugin -saveAs supergene.phy -format Phylip_Inter
请问老师,在构建进化树时第一步排序是否是必须的?