提示文件路径不对,你检查变量对应的文件路径是否正确
应该如何解决?代码与报错如下,数据来源:ncbi的参考基因组注释文件。
/share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl --gff3 $species.longest_isoform.gff3 --fasta $genome --seqType CDS >$species.cds.fa
#提取pep序列
/share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl --gff3 $species.longest_isoform.gff3 --fasta $genome --seqType prot >$species.pep.fa
报错如下:
No such file or directory at /share/work/biosoft/TransDecoder/TransDecoder-v5.5.0/util/../PerlLib/GFF3_utils2.pm line 40, <$filehandle> line 3167.
No such file or directory at /share/work/biosoft/TransDecoder/TransDecoder-v5.5.0/util/../PerlLib/GFF3_utils2.pm line 40, <$filehandle> line 3167.