Use of uninitialized value $1 in hash element at /mnt/data/home/tycloud/annovar/annovar/2019Oct24/annotate_variation.pl line 1445, <$QUERY> line 3623855.
NOTICE: Finished analyzing 2000000 query variants
Use of uninitialized value $1 in hash element at /mnt/data/home/tycloud/annovar/annovar/2019Oct24/annotate_variation.pl line 1445, <$QUERY> line 3623855.
NOTICE: Finished analyzing 3000000 query variants
NOTICE: Reading FASTA sequences from /mnt/data/home/tycloud/my_reseq/ref/unknown_refGeneMrna.fa ... Done with 20994 sequences
WARNING: A total of 49 sequences will be ignored due to lack of correct ORF annotation
NOTICE: Running with system command <coding_change.pl /mnt/data/home/tycloud/my_reseq/annovar/snp.refGene.exonic_variant_function.orig /mnt/data/home/tycloud/my_reseq/ref/unknown_refGene.txt /mnt/data/home/tycloud/my_reseq/ref/unknown_refGeneMrna.fa -alltranscript -out /mnt/data/home/tycloud/my_reseq/annovar/snp.refGene.fa -newevf /mnt/data/home/tycloud/my_reseq/annovar/snp.refGene.exonic_variant_function>
Error: invalid record found in exonic_variant_function file (exonic format error):
这是不是软件版本问题啊?老师,很奇怪,上个月用的时候还可以运行,这个月一样报错,输入文件是同一个vcf文件,应该可以排除输入文件的问题。