braker.log 最后为什么会删除augustus结果。我的运行命令为braker.pl --species=$species \
--genome=$contig \
--softmasking \
--bam=rnaseq.bam \
--cores=$threads \
--useexisting \
--gff3 --skip_fixing_broken_genes
# Tue Jan 16 17:02:31 2024: Did not find environment variable $AUGUSTUS_BIN_PATH (either variable does not exist, or the path given in variable does not exist). Will try to set this variable in a different way, later.
# Tue Jan 16 17:02:31 2024: Trying to guess $AUGUSTUS_BIN_PATH from $AUGUSTUS_CONFIG_PATH.
# Tue Jan 16 17:02:31 2024: Setting $AUGUSTUS_BIN_PATH to /share/work/biosoft/augustus/latest/config//../bin
# Tue Jan 16 17:02:31 2024: Did not find environment variable $AUGUSTUS_SCRIPTS_PATH (either variable does not exist, or the path given in variable does not exist). Will try to set this variable in a different way, later.
# Tue Jan 16 17:02:31 2024: Trying to guess $AUGUSTUS_SCRIPTS_PATH from $AUGUSTUS_CONFIG_PATH.
# Tue Jan 16 17:02:31 2024: Setting $AUGUSTUS_SCRIPTS_PATH to /share/work/biosoft/augustus/latest/config//../scripts
# Tue Jan 16 17:02:31 2024: Did not find environment variable $PYTHON3_PATH
# Tue Jan 16 17:02:31 2024: Trying to guess $PYTHON3_PATH from location of python3 executable that is available in your $PATH
# Tue Jan 16 17:02:31 2024: Setting $PYTHON3_PATH to /share/work/biosoft/python/Python3/bin
# Tue Jan 16 17:02:31 2024: Found environment variable $GENEMARK_PATH. Setting $GENEMARK_PATH to /share/work/biosoft/GeneMark-ES/latest/gmes_linux_64_4/
# Tue Jan 16 17:02:31 2024: Did not find environment variable $BAMTOOLS_PATH (either variable does not exist, or the path given in variable does not exist). Will try to set this variable in a different way, later.
# Tue Jan 16 17:02:31 2024: Trying to guess $BAMTOOLS_BIN_PATH from location of bamtools executable that is available in your $PATH
# Tue Jan 16 17:02:31 2024: Setting $BAMTOOLS_BIN_PATH to /share/work/biosoft/bamtools/latest/bin
# Tue Jan 16 17:02:31 2024: Did not find environment variable $SAMTOOLS_PATH (either variable does not exist, or the path given in variable doesnot exist). Will try to set this variable in a different way, later.
# Tue Jan 16 17:02:31 2024: Trying to guess $SAMTOOLS_PATH from location of samtools executable in your $PATH
# Tue Jan 16 17:02:31 2024: Setting $SAMTOOLS_PATH to /share/work/biosoft/samtools/latest/bin
# Tue Jan 16 17:02:31 2024: Did not find environment variable $DIAMOND_PATH
# Tue Jan 16 17:02:31 2024: Trying to guess $DIAMOND_PATH from location of diamond executable that is available in your $PATH
# Tue Jan 16 17:02:31 2024: Setting $DIAMOND_PATH to /share/work/biosoft/toolsbraker.log.txt
braker.log 最后为什么会删除augustus结果。我的运行命令为braker.pl --species=$species \
--genome=$contig \
--softmasking \
--bam=rnaseq.bam \
--cores=$threads \
--useexisting \
--gff3 --skip_fixing_broken_genes