拼接好的数据,可以作为单端输入,准备se-33-manifest:
sample-id absolute-filepath sample-1 $PWD/some/filepath/sample1_R1.fastq sample-2 $PWD/some/filepath/sample2_R1.fastq
导入命令:
qiimetoolsimport\ --type'SampleData[SequencesWithQuality]'\ --input-pathse-33-manifest\ --output-pathsingle-end-demux.qza\ --input-formatSingleEndFastqManifestPhred33V2
参考:https://docs.qiime2.org/2024.2/tutorials/importing/