这个shell变量是不是没有 export设置正确,再看看视频吧:datadir
There was a problem importing /work/omicsclass/ampliseq-q2/qiime2_2022.8/data_2/manifest.ckd.txt:
/work/omicsclass/ampliseq-q2/qiime2_2022.8/data_2/manifest.ckd.txt is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 3 and column "forward-absolute-filepath" could not be found ($datadir/AWGB16SAA2134120-38-28_R1.fq.gz) for sample "AWGB16SAA2134132-38-28".