输入的数据可能有问题,纠错步骤抱错了。
再看看计算资源是否够,内存是不是够;
安装教程里的代码运行,使用unicycler软件时报错,报错信息如下
== Error == system call for: "['/share/work/biosoft/SPAdes/SPAdes-3.15.4-Linux/bin/spades-core', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/config.info', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/isolate_mode.info']" finished abnormally, OS return value: -9
None
======= SPAdes pipeline finished abnormally and WITH WARNINGS!
=== Error correction and assembling warnings:
* 0:06:44.998 1783M / 18G WARN General (kmer_coverage_model.cpp : 218) Too many erroneous kmers, the estimates might be unreliable
* 0:07:10.518 1785M / 18G WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 3
* 0:07:10.523 1785M / 18G WARN General (kmer_coverage_model.cpp : 366) Failed to determine erroneous kmer threshold. Threshold set to: 3
======= Warnings saved to /work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/warnings.log
=== ERRORs:
* system call for: "['/share/work/biosoft/SPAdes/SPAdes-3.15.4-Linux/bin/spades-core', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/config.info', '/work/my_mt/01.plant_assemble/21.mapped_reads/22.unicycler/spades_assembly/K27/configs/isolate_mode.info']" finished abnormally, OS return value: -9