是使用的我们的课程代码吗:https://bdtcd.xetslk.com/s/qZRiF
如果是的,你这个输入文件( $dbdir/qiime2/unite_ver9_99_25.07.2023-Q2-2023.7.qza)写错了,脚本里面不是这个文件
# 有参考序列,目前unite没有下载到最新的参考序列
qiime deblur denoise-other \
--i-demultiplexed-seqs demux-joined-filtered.qza \
--i-reference-seqs $dbdir/qiime2/unite_ver9_99_25.07.2023-Q2-2023.7.qza \
--p-trim-length -1 \
--p-sample-stats \
--p-jobs-to-start 10 \
--p-min-reads 1 \
--o-representative-sequences deblur-repset-seqs.qza \
--o-table deblur-table.qza \
--o-stats deblur-stats.qza
报错如下
There was a problem with the command:
(1/1) Invalid value for '--i-reference-seqs': Expected an artifact of at
least type FeatureData[Sequence]. An artifact of type TaxonomicClassifier
was provided.