如何用荧光定量数据绘制热图？
这是看到的一篇文献中Fig. 6. Expression profiles of PtSAUR genes in different tissues as determined by qRT-PCR.
There were three biological and three technical
replicates performed for each sample. The relative expression levels
were evaluated by 2-ΔΔCT. GraphPad software was used for the statistical analyses.
作者是用的2-ΔΔCT的方法做的数据分析，可是结果怎么会是负数呢？
在问答社区内看到
莫非他是用的-ΔΔCT？恳请老师们解答，谢谢了
最佳答案 2019-10-29 15:36
为了是热图每一行对比明细，使热图很好看，热图一般按行均一化数据。所以，legend会有负数。聚类热图绘制见：https://www.omicsclass.com/article/528
qpcr的计算方法见:https://www.omicsclass.com/article/446
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