使用tcga_gene_exp_download.r下载TCGA数据始终无法产生gene expression相关的文件 killed

attachments-2021-10-DO7C4D6961687079424fb.png
这是我用docker 镜像下载完成后的数据,更换成课程使用的HNSC也无法产生表达的总结数据,更换网络也不能改善

更换网络同样无法改善

attachments-2021-10-nJYvvEFk6168709175ac7.png

代码没做太多修改,是什么参数除了问题吗

表达的原始数据应该成功下载

attachments-2021-10-aZNpe1vq616870675cd1a.png运行过程:

Failed to create bus connection: No such file or directory

[1] 608  66

--------------------------------------

o GDCquery: Searching in GDC database

--------------------------------------

Genome of reference: hg38

--------------------------------------------

oo Accessing GDC. This might take a while...

--------------------------------------------

ooo Project: TCGA-OV

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oo Filtering results

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ooo By file.type

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oo Checking data

----------------

ooo Check if there are duplicated cases

Warning: There are more than one file for the same case. Please verify query results. You can use the command View(getResults(query)) in rstudio

ooo Check if there results for the query

-------------------

o Preparing output

-------------------

Downloading data for project TCGA-OV

GDCdownload will download 590 files. A total of 31.440651 MB

Downloading chunk 1 of 59 (10 files, size = 600.677 KB) as Sun_Oct_17_23_40_26_2021_0.tar.gz

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  |======================================================================| 100%

Parsing follow up version: follow_up_v1.0

  |======================================================================| 100%

  |======================================================================|  99%

  |======================================================================| 100%

To get the following information please change the clinical.info argument

=> new_tumor_events: new_tumor_event 

=> drugs: drug 

=> follow_ups: follow_up 

=> radiations: radiation

Parsing follow up version: follow_up_v1.0

  |======================================================================| 100%

Adding stage event information

  |======================================================================| 100%

  |======================================================================| 100%

  |======================================================================| 100%

  |======================================================================| 100%

Warning message:

`funs()` was deprecated in dplyr 0.8.0.

Please use a list of either functions or lambdas: 


  # Simple named list: 

  list(mean = mean, median = median)


  # Auto named with `tibble::lst()`: 

  tibble::lst(mean, median)


  # Using lambdas

  list(~ mean(., trim = .2), ~ median(., na.rm = TRUE))

This warning is displayed once every 8 hours.

Call `lifecycle::last_warnings()` to see where this warning was generated. 

--------------------------------------

o GDCquery: Searching in GDC database

--------------------------------------

Genome of reference: hg38

--------------------------------------------

oo Accessing GDC. This might take a while...

--------------------------------------------

ooo Project: TCGA-OV

--------------------

oo Filtering results

--------------------

ooo By data.type

ooo By workflow.type

----------------

oo Checking data

----------------

ooo Check if there are duplicated cases

ooo Check if there results for the query

-------------------

o Preparing output

-------------------

Total files to download: 379Total TP samples to down: 374Total NT samples to down: 0Downloading data for project TCGA-OV

GDCdownload will download 379 files. A total of 97.709866 MB

Downloading chunk 1 of 38 (10 files, size = 2.572636 MB) as Sun_Oct_17_23_47_13_2021_0.tar.gz

Downloading: 750 kB     At least one of the chunks download was not correct. We will retry

Downloading chunk 1 of 38 (10 files, size = 2.572636 MB) as Sun_Oct_17_23_47_13_2021_0.tar.gz

Downloading: 2.6 MB     Downloading chunk 2 of 38 (10 files, size = 2.579897 MB) as Sun_Oct_17_23_47_13_2021_1.tar.gz

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|====================================================|100%                      Completed after 29 s 

Killed

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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

运行的过程中报什么错了了,可以截图一下吗?


再个,这个脚本会整理数据,电脑的内存太少会导致内存不够,从而运行失败; 建议最低 至少16G以上  32G为佳

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  • 一个正常的ID 提出于 2021-10-15 02:02

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