具体报错的信息如下
Traceback (most recent call last):
File "/biosoft/miniconda/envs/qiime1/bin/pick_open_reference_otus.py", line 453, in <module>
main()
File "/biosoft/miniconda/envs/qiime1/bin/pick_open_reference_otus.py", line 432, in main
minimum_failure_threshold=minimum_failure_threshold)
File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/pick_open_reference_otus.py", line 1030, in pick_subsampled_open_reference_otus
status_update_callback=status_update_callback)
File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/pick_open_reference_otus.py", line 232, in assign_tax
close_logger_on_success=close_logger_on_success)
File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/util.py", line 122, in call_commands_serially
raise WorkflowError(msg)
qiime.workflow.util.WorkflowError:
*** ERROR RAISED DURING STEP: Assign taxonomy
Command run was:
assign_taxonomy.py -o pick_open_reference_otus/uclust_assigned_taxonomy -i pick_open_reference_otus/rep_set.fna --reference_seqs_fp /work/database/SILVA_132_QIIME_release/rep_set/rep_set_16S_only/97/silva_132_97_16S.fna --id_to_taxonomy_fp /work/database/SILVA_132_QIIME_release/taxonomy/16S_only/97/taxonomy_7_levels.txt --assignment_method uclust --similarity 0.8
Command returned exit status: 1
Stdout:
Stderr
sh: line 1: 556 Segmentation fault uclust --input "pick_open_reference_otus/rep_set.fna" --id 0.8 --rev --maxaccepts 3 --allhits --libonly --lib "/work/database/SILVA_132_QIIME_release/rep_set/rep_set_16S_only/97/silva_132_97_16S.fna" --uc "/work/tmp/UclustConsensusTaxonAssigner_3che8T.uc" > "/work/tmp/tmpcfK94YiqcvPEpOq0MYbL.txt" 2> "/work/tmp/tmpYU1idAfaqTFEbQtQEBGJ.txt"
Traceback (most recent call last):
File "/biosoft/miniconda/envs/qiime1/bin/assign_taxonomy.py", line 417, in <module>
main()
File "/biosoft/miniconda/envs/qiime1/bin/assign_taxonomy.py", line 394, in main
log_path=log_path)
File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/assign_taxonomy.py", line 1304, in __call__
'--uc': uc_path})
File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/burrito/util.py", line 285, in __call__
'StdErr:\n%s\n' % open(errfile).read())
burrito.util.ApplicationError: Unacceptable application exit status: 139
Command:
cd "/work/mylunwen/5.pick_otu_qiime/"; uclust --input "pick_open_reference_otus/rep_set.fna" --id 0.8 --rev --maxaccepts 3 --allhits --libonly --lib "/work/database/SILVA_132_QIIME_release/rep_set/rep_set_16S_only/97/silva_132_97_16S.fna" --uc "/work/tmp/UclustConsensusTaxonAssigner_3che8T.uc" > "/work/tmp/tmpcfK94YiqcvPEpOq0MYbL.txt" 2> "/work/tmp/tmpYU1idAfaqTFEbQtQEBGJ.txt"
StdOut:
StdErr:
