docker运行qiime1时按照教程进行到章节2课时6时，即聚类生成OTU-denovo时老是报错，查看类似问题后，升级了运行内存为70g，同时将教程内的30多个样本减成2个样本，FASTQ格式还有qimme格式检查都正确，依旧报错，报错内容如下：

pick_de_novo_otus.py -i qiime.fasta -f -o pick_de_novo_otus \

>     -p otu_params_de_novo.txt

/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/matplotlib/font_manager.py:273: UserWarning: Matplotlib is building the font cache using fc-list. This may take a moment.

  warnings.warn('Matplotlib is building the font cache using fc-list. This may take a moment.')

Traceback (most recent call last):

  File "/biosoft/miniconda/envs/qiime1/bin/pick_de_novo_otus.py", line 180, in <module>

    main()

  File "/biosoft/miniconda/envs/qiime1/bin/pick_de_novo_otus.py", line 177, in main

    status_update_callback=status_update_callback)

  File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/upstream.py", line 306, in run_pick_de_novo_otus

    close_logger_on_success=close_logger_on_success)

  File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/workflow/util.py", line 122, in call_commands_serially

    raise WorkflowError(msg)

qiime.workflow.util.WorkflowError:

*** ERROR RAISED DURING STEP: Assign taxonomy

Command run was:

 assign_taxonomy.py -o pick_de_novo_otus/uclust_assigned_taxonomy -i pick_de_novo_otus/rep_set//qiime_rep_set.fasta --reference_seqs_fp /work/database/SILVA_132_QIIME_release/rep_set/rep_set_16S_only/97/silva_132_97_16S.fna --id_to_taxonomy_fp /work/database/SILVA_132_QIIME_release/taxonomy/16S_only/97/taxonomy_7_levels.txt --assignment_method uclust --similarity 0.8

Command returned exit status: 1

Stdout:

Stderr

sh: line 1:    98 Segmentation fault      uclust --input "pick_de_novo_otus/rep_set//qiime_rep_set.fasta" --id 0.8 --rev --maxaccepts 3 --allhits --libonly --lib "/work/database/SILVA_132_QIIME_release/rep_set/rep_set_16S_only/97/silva_132_97_16S.fna" --uc "/work/tmp/UclustConsensusTaxonAssigner_jC5NV4.uc" > "/work/tmp/tmpsnWnyn7pct7xAzj5h5pm.txt" 2> "/work/tmp/tmpHGfUbEEXt29bAiXMuOoV.txt"

Traceback (most recent call last):

  File "/biosoft/miniconda/envs/qiime1/bin/assign_taxonomy.py", line 417, in <module>

    main()

  File "/biosoft/miniconda/envs/qiime1/bin/assign_taxonomy.py", line 394, in main

    log_path=log_path)

  File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/qiime/assign_taxonomy.py", line 1304, in __call__

    '--uc': uc_path})

  File "/biosoft/miniconda/envs/qiime1/lib/python2.7/site-packages/burrito/util.py", line 285, in __call__

    'StdErr:\n%s\n' % open(errfile).read())

burrito.util.ApplicationError: Unacceptable application exit status: 139

Command:

cd "/work/my_16s/5.pick_otu_qiime/"; uclust --input "pick_de_novo_otus/rep_set//qiime_rep_set.fasta" --id 0.8 --rev --maxaccepts 3 --allhits --libonly --lib "/work/database/SILVA_132_QIIME_release/rep_set/rep_set_16S_only/97/silva_132_97_16S.fna" --uc "/work/tmp/UclustConsensusTaxonAssigner_jC5NV4.uc" > "/work/tmp/tmpsnWnyn7pct7xAzj5h5pm.txt" 2> "/work/tmp/tmpHGfUbEEXt29bAiXMuOoV.txt"

StdOut:

StdErr: