我通过GeSeq进行注释后,用Geneious进行检查并手动纠错后,删掉了info和annotation行后,将这个库重新用CPGAVAS2进行注释,就会报下面的错说“no hit found”,请问是什么原因呢?
我的部分注释如下:
gene complement(4911..4984)
/gene="trnP-UGG"
tRNA complement(4911..4984)
/gene="trnP-UGG"
gene complement(5042..5566)
/gene="ORF174"
CDS complement(5042..5566)
/gene="ORF174"
/codon_start=1
/transl_table=11
/translation="MIFWKKFFQNSDSNTSSEKIFKKLIVLDKAFDSIEFKDIYLSST
KNINLYELEQLCDSVGWVKRPLKKVKIALKNSSIIISLIQKKDANTKLVGFARATSDN
GFNATIWDVVIHPDFQGLGLGKVVIHQLIQQLRQAEISTITLFAEPDVVSFYKKLGFI
KDPDGVKGMFWYPR"
gene 5845..6390
/gene="psaF"
CDS 5845..6390
/gene="psaF"
/codon_start=1
/transl_table=11
/translation="MFLNCLLALLLLSNPIIANAEVAGLVPCKDSAAFNKRMVNSVKK
LQTRLAKYDADTPPALALNKQIEKTKTRFATYGRAGLLCGTDGLPHLISDGRWSRAGD
FVFPGLLFLYITGWIGWVGRGYLLSVAKTSKPTEKEIILDVPLAIKFMSSGFAWPLAA
WQEFSSGQLIASNDDITVSPR"
gene 6403..6531
/gene="psaJ"
CDS 6403..6531
/gene="psaJ"
/codon_start=1
/transl_table=11
/translation="MNNNFTKYLSTAPVIGVLWMTFTAGFIIELNRFFPDVLYFYL"
gene 6688..7173
/gene="apcD"
CDS 6688..7173
/gene="apcD"
/codon_start=1
/transl_table=11
/translation="MSLVSQIIVNADDELRYPTIGELQSIQDYLITGSNRIRIATIIR
DKEKEIIQKASKQIFQLHPEYIAPGGNAAGSRKRSLCLRDYGWYLRLITYGVLAGDKD
SIETIGIIGVREMYNSLGVPIIGMLDAIQCLKEASLEMLDQDDIKIIAPYFDYIIRGM
S"
gene 7510..8487
/gene="fabH"
CDS 7510..8487
/gene="fabH"
/codon_start=1
/transl_table=11
/translation="MGVHILSTGSSVPNFSVENQQFEDIIETSDHWISTRTGIKKRHL
APSSTSLTKLAAEAANNALCAANLKPTDINLIILATSTPDDLFGSASQLQAEIGATAS
VAFDITAACSGFIIALVTATQFIQTGSYDNILVVGADTMSRWINWSDRTTCILFGDGA
GAVVLRQSLKNSILGFKLCTDGQLNSHLQLMNTPVNNQKFGVTEIPHGNYNSITMNGK
EVYKFAVFQVPTVIRQCLNNLNISIDEVDWFILHQANTRILEAIASRLSIPFSKMITN
LENYGNTSAASIPLALDEAIKSNKIQPGQTIVLSGFGAGLTWGAIVLKW"
gene complement(8496..8579)
/gene="trnL-GAG"
tRNA complement(8496..8579)
/gene="trnL-GAG"
gene 8751..8870
/gene="psbX"
CDS 8751..8870
/gene="psbX"
/codon_start=1
/transl_table=11
/translation="MTPSLSSFLNSLILGAVIVVVPITLALLFVSQKDRTIRS"
gene 9148..10014
/gene="accD"
CDS 9148..10014
/gene="accD"
/codon_start=1
/transl_table=11
/translation="MSLSNWPLKKENSEAYTIKNSKQITIPDGLWIKCFDCGLLMYSK
VLKRNLKVCPQCSYHFQASSNERIDQLIDQGSWQPMDVHLISTDPLGFKDQKLYSQRL
KDTALKTGLQDAVQTGIGTMQGRQVCLGIMDFRFMGGSMGSVVGEKLTRLLEKATQEK
LPAIILCASGGARMQEGMLSLMQMAKISSALEMHKKANLLYISVLTSPTTGGVTASFA
MLGDLIIAEPKALIAFAGRRVIEQTIKEDLPDNFQSSEYLFEHGFLDLIVPRTQLRSK
LIQILSLHTHSK"
gene complement(10064..10149)
/gene="trnA-1"
tRNA complement(10064..10149)
/gene="trnA-1"
gene 10288..10779
/gene="psbV"
CDS 10288..10779
/gene="psbV"
/codon_start=1
/transl_table=11
/translation="MLKRSSWLAALLGLLTIVSTSTHAHAIELDEATRTVPLESSGKT
VVLTPEQVKRGKRLFNNSCAICHNGGITKTNPNIGLDPESLGLATPQRDTIEGLVDYM
KDPTSYDGAESIAELHPSIKSAEIFPKMRNLTDEDLFTIAGHILLQPKIVSEKWGGGK
IYY"
gene 10950..11282
/gene="petJ"
CDS 10950..11282
/gene="petJ"
/exception="RNA editing"
/translation="MKKTLSVLFTVFSFFVIGFAQIAFAADLDNGEKVFSANCAACHA
GGNNAIMPDKTLKKDVLEANSMNSIDAITYQVKNGKNAMPAFGGRLVDEDIEDAANYV
LSQSEKGW"
gene 11396..12550
/gene="carA"
CDS 11396..12550
/gene="carA"
/codon_start=1
/transl_table=11
/translation="MMKKIPAILVLQDGTYYKGWSFQQEKQVITIGEVVFNTGMTGYQ
EIITDPSYFQQIVTFTYPEIGNTGINNQDIESNSISIKGLVAKNICKISSSWREQESL
VKYLSSHDIPFIFGIDTRSLTQYLRQFGTMNGCISTDSLNHSYLKQKISEIPGMQGLD
LIPYVTTGSVYPWDEKSLPNWYLTDRIRVQLVIQLKVVVIDFGVKLNILRRLATFGCQ
ITVVPAQTSLEDILAYQPDGILLSNGPGDPSAVHYGIQTVTNLLDHNVPIFGICMGHQ
ILNLALKAKTFKLKFGHRGINHPSGLNQQVEITSQNHGFAVDFYSVLESSIKVTHFNL
NDTTVAGTGHNQSPYFSVQYHPESSPGPHDADYLFENFVEIMTKSKQKVS"