发不了图,我粘一下文字,最后提示好像是路径的问题?我反复对比视频内容发现视频里的镜像omicsclass/reseq是8.76G,目前镜像更新后的版本是13.8G,会不会是镜像更新后出现了Bug,希望能够得到解答,错误提示在文字最后面。
SEQUENCE_TARGET=201,10
SPRIMER_TASK=pick_detection_primers
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_NUM_RETURN=1
PRIMER_PRODUCT_OPT_SIZE=125
PRIMER_MAX_END_STABILITY=250
PRIMER_MIN_SIZE=18
PRIMER_OPT_SIZE=20
PRIMER_MAX_SIZE=23
PRIMER_MAX_NS_ACCEPTED=1
PRIMER_MIN_GC=40.0
PRIMER_MAX_GC=60.0
PRIMER_PRODUCT_SIZE_RANGE=100-300
SEQUENCE_INTERNAL_EXCLUDED_REGION=201,10
PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not specified
=