使用命令“braker.pl --species=$species    --genome=$contig    --softmasking    --bam=rnaseq.bam    --cores=$threads    --useexisting      --gff3 --skip_fixing_broken_genes”

报错“# Mon Aug 12 20:10:06 2024: Log information is stored in file /work/work/xsyj/genome_annotation/03.Braker/braker/braker.log

ERROR in file /share/work/biosoft/BRAKER/latest/scripts//braker.pl at line 6666

Failed to execute: perl /share/work/biosoft/GeneMark-ES/latest/gmes_linux_64_4//gmes_petap.pl --verbose --sequence=/work/work/genome_annotation/03.Braker/braker/genome.fa --ET=/work/work/genome_annotation/03.Braker/braker/genemark_hintsfile.gff --cores=15 --gc_donor 0.001 --soft_mask auto 1>/work/work/genome_annotation/03.Braker/braker/GeneMark-ET.stdout 2>/work/work/genome_annotation/03.Braker/braker/errors/GeneMark-ET.stderr

Failed to execute: perl /share/work/biosoft/GeneMark-ES/latest/gmes_linux_64_4//gmes_petap.pl --verbose --sequence=/work/work/genome_annotation/03.Braker/braker/genome.fa --ET=/work/work/xsyj/genome_annotation/03.Braker/braker/genemark_hintsfile.gff --cores=15 --gc_donor 0.001 --soft_mask auto 1>/work/work/genome_annotation/03.Braker/braker/GeneMark-ET.stdout 2>/work/work/genome_annotation/03.Braker/braker/errors/GeneMark-ET.stderr

The most common problem is an expired or not present file ~/.gm_key!”

更新了gm_key文件还是同样的报错