具体的错误提示如下:
****************************************************************
Checking input parameters: [OK]
Checking Epal2nal.pl: [OK]
Checking multiple sequence aligner: muscle[OK]
Checking output format: [OK]
Checking output folder: [OK]
Outputing input parameters:
Homologous groups = homo_pairs.txt1
Amino acids sequences = Input_pep.fa
Nucleotide sequences = Input_cds.fa
Process file = proc.txt
Output folder = align_out
Multiple sequence aligner = muscle
Output format = axt
Genetic code = 1-The Standard Code
KaKs_Calculator used = FALSE
Remove gap = FALSE
Remove mismatched codons = FALSE
Verbose output = FALSE
Reading homologous groups from homo_pairs.txt1: 100 groups
Reading nucleotide sequences from Input_cds.fa: 118339 nucleotide sequences
Reading amino acid sequences from Input_pep.fa: 118339 amino acid sequences
Generating homologous group files for alignments: 0 from 100 groups
Aligning amino acid sequences for 0 homologous groups:
Translating amino acid alignments into codon alignments:
Cleaning temporary files:
Mission Accomplished (Time used: 0s).