老师您好,请问做BWA比对时与参考基因组的比对率很低,已经确定过与参考基因组为同一物种,是因为污染太严重吗,这样会对后续分析有影响吗?
38280780 + 0 primary
616640 + 0 secondary
0 + 0 supplementary
4716288 + 0 duplicates
4716288 + 0 primary duplicates
20943404 + 0 mapped (53.84% : N/A)
20326764 + 0 primary mapped (53.10% : N/A)
38280780 + 0 paired in sequencing
19140390 + 0 read1
19140390 + 0 read2
17205178 + 0 properly paired (44.94% : N/A)
18888798 + 0 with itself and mate mapped
1437966 + 0 singletons (3.76% : N/A)
1344176 + 0 with mate mapped to a different chr
923040 + 0 with mate mapped to a different chr (mapQ>=5)